P-791 Supplementation of mitochondria from endometrial mesenchymal stem cells (EnMSCs) improves oocyte quality in aged mice

Abstract Study question To improve the quality of aging oocytes by supplementing mitochondria from endometrial mesenchymal stem cells (EnMSCs) via microinjection. Summary answer Supplementation with EnMSC-derived can and promote embryo development in mice. What is known already Maternal one major causes reduced ovarian reserve low oocyte elderly women. Decreased main cause age-related infertility. Mitochondria are multifunctional energy stations that determine quality. The display functional impairments mtDNA damage, which leads to competence developmental potential oocytes. quality, mitochondrial supplementation carried out as a therapeutic approach. However, selection suitable source remains controversial. design, size, duration design: Eight-month-old ICR mice were purchased then further raised ten months age for use experiments. EnMSCs stably cultivated extracted ten-month-old size: About 300 GV 134 MII counted . 80 fertilized transplanted into fallopian tubes surrogate mothers. duraton: half year. Participants/materials, setting, methods Participants:ten-month-old Setting methods: We aged EnMSCs. And supplemented assess effects on development, ICSI combining microinjection.To test whether could vivo mice, transplantation was performed after microinjection combined ICSI. Main results role chance In this study, we found derived significantly oocytes.Supplementation EnMSC increased blastocyst ratio intracytoplasmic sperm injection (ICSI). also birth rate mitochondria-injected transplantation. Limitations, reasons caution Microinjection invasive some died ability method limited because 35.65±1.75% while 6–8 weeks previous study nearly 80%. Wider implications findings For clinical treatment, be obtained patient's own menstrual blood sources supplementation. A noninvasive procedure cell extraction ensures safety, work might provide valuable strategy patients declining due aging. Trial registration number Not applicable